Genospecies D
The sister group of gsE looks like this:
There is a tight clade that includes the 5 strains from clover in Denmark that were identified as genospecies D in the study by Cavassim et al. (2020, https://doi.org/10.1099/mgen.0.000351), as well as WSM80 and WSM448 (from unspecified root nodules in Australia) and L145 (lentil in France). Then there are two strains, CC278f (clover in USA) and Norway (Lotus in Norway) that are more distant from this group and from each other.
The ANI plot for all strains in the Rlc looks like this, using SM51 as the reference strain: 
The lowest ANI in the tight clade is 98.77, whereas ANI for Norway is 95.62 and for CC278f 94.99 (lower than for some gsE strains). Although the phylogeny places them on the gsD branch, these two outlying strains have similar ANI to gsE as to gsD. Against the gsE reference USDA2370, ANI for Norway is 94.54 and for CC278f it is 95.00. Between the two strains, ANI is 95.81 or 95.74 (depending on direction). I conclude that these two strains cannot be placed in gsD or gsE, and possibly represent two separate species.
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